Cellular Imaging Instrumentation Accessories
Sartorius Incucyte 3D Nanowell Plate
The Incucyte 3D Nanowell Plates facilitate high-throughput organoid studies by creating a 3D microenvironment that mimics in vivo conditions supporting organoid growth and differentiation. These plates feature an array of hydrogel-printed microwells each optimized to foster uniform and reproducible organoid formation. Additionally each well contains a dedicated pipetting port for seamless medium changes that can be integrated with automated processes thus enhancing efficiency and reproducibility in large-scale screening assays. Together with the Incucyte Live-Cell Analysis System and Incucyte Organoid Analysis Software Module 9600-0034 these plates enable high-throughput long-term imaging and analysis of organoids providing insights into their growth and viability dynamics. This plate also requires systems to be operating on Incucyte Base Software v2024A or later.
Sigma Aldrich Fine Chemicals Biosciences 3dGROTM Lung Organoid Bran
The lung epithelium undergoes a complex series of endodermndashmesoderm-mediated signaling events to generate a complex arborized network of conducting airway (bronchi bronchioles) and gas-exchanging units (alveoli). Lung epithelium differentiation stages include anterior/posterior and dorsal/ventral patterning lung specification lung budding branching morphogenesis and maturation. The 3dGRO(TM) Lung Organoid Branching Medium is a chemically defined/serum-free ready-to-use media used to differentiate Sox2/Pax6 anterior foregut endoderm (AfE) cells into lung bud organoids in suspension cultures. Additionally the 3dGRO(TM) Lung Organoid Branching Medium can be used to culture AfE cells on fibronectin coated cultureware. The 3dGRO(TM) Lung Organoid Branching Medium is a component of the 3dGRO(TM) Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar s
Sartorius Incucyte Neuroactive Kit
The Incucyte Neuroactive Kit contain cryopreserved vials of rat cortical neurons and astrocytes isolated from embryonic stage E18 Sprague-Dawley rats as well as sufficient Incucyte Neuroburst Orange Lentivirus to perform one 96-well experiment.The Incucyte Neuroburst Orange Lentivirus is a lentiviral based live-cell neuronal labeling reagent driven by a synapsin promoter resulting in the long term expression of a genetically encoded orange fluorescent calcium indicator mRuby-based in neuronal cells.
INTACT GENOMICS INC ig Recombinase Polymerase Amplification (RPA) Kit v2, 25 Reactions
The improved IG Recombinase Polymerase Amplification Version 2 Kit (IG RPA Kit v2) amplifies DNA at a single and constant temperature (25°C – 42°C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX is used in combination with its accessory protein, UvsY. The recombinase interacts with the primers to form nucleoprotein filaments. This complex can bind with homologous double-stranded DNA through a strand exchange. After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu/Sau DNA polymerase extends the DNA from the primers, creating a new complete copy of the template, and amplification can continue as in Polymerase Chain Reaction (PCR). IG RPA v2 offers More robust amplification and more specific amplification of the primary target than Version 1 for some applications. It may reduce background amplification of off-target sites.
